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Part:BBa_K1702003:Design

Designed by: Olivia Smith   Group: iGEM15_CSU_Fort_Collins   (2015-09-17)


Lipase L1 from Bacillus stearothermophilus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 481
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 707


Design Notes

Expression of this gene is inducible with lactose or IPTG.


Source

This part was synthesized based on a sequence from Kim, Hyung-Kwoun et al. "Gene Cloning and Characterization of Thermostable Lipase from Bacillus stearothermophilus L1." Bioscience, Biotechnology, and Biochemistry 62(1):66-71. 1998.

References